fast protein liquid chromatography fplc system (Cytiva Europe)
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fast protein liquid chromatography fplc system
Fast Protein Liquid Chromatography Fplc System, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 3269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fast+protein+liquid+chromatography+fplc+system/AKTA+pure/pm41723978-40-22-30
Average 98 stars, based on 3269 article reviews
Fast Protein Liquid Chromatography Fplc System, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 3269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fast+protein+liquid+chromatography+fplc+system/AKTA+pure/pm41723978-40-22-30
Average 98 stars, based on 3269 article reviews
fast protein liquid chromatography fplc system - by Bioz Stars,
2026-10
98/100 stars
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Fast Protein Liquid Chromatography:Article Title: Hazelnut peptide fractions preserve their bioactivities beyond industrial manufacture and simulated digestion of hazelnut cocoa cream. Article Snippet: Cold press hazelnut cakes represent a concentrated source of proteins that can be industrially exploited.. Previously, bioactive attributes of hazelnut protein hydrolysates including antihypertensive and antidiabetic activities were documented.. Here, we made an attempt to utilize bioactive hazelnut protein hydrolysates (1 % w/w) in the manufacture of industrial hazelnut cocoa cream and investigate their stability through processing and simulated gastrointestinal digestion. Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania Article Snippet: Cells were harvested by centrifugation, and the pellet was resuspended in 10 mL lysis buffer containing 500 mM KCl, 20 mM Hepes, 5 mM imidazole, pH 7.5 and protease inhibitor cocktail without EDTA (Merck). .. After lysis by sonication (20 sec, 4 times), the soluble fraction was obtained by centrifugation (30,000 x g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. Article Snippet: purification Cas9 The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector, which contains two N- terminal and C- terminal His- tag (Gift from Fanny Boissier, INSERM U1212 CNRS 5320, University of Bordeaux), using the NcoI and EcoRI restriction sites. .. After lysis by sonication (20 sec, four times), the soluble fraction was obtained by centrifugation (30,000× g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His- Select Nickel Affinity Gel (Sigma) in a Article Title: One-Pot Three-Enzyme System for Production of a Novel Prebiotic Mannosyl-β-(1 → 4)-Fructose Using a d-Mannose Isomerase from Xanthomonas phaseoli . Article Snippet: The present supply of prebiotics is entirely inadequate to meet their demand.. To produce novel prebiotics, a Dmannose isomerase (XpMIaseA) from Xanthomonas phaseoli was first produced in Komagataella phaffii (Pichia pastoris).. XpMIaseA shared the highest amino acid sequence identity (58.0%) with the enzyme from Marinomonas mediterranea. Article Title: Evaluation of the precision of the Plasmodium knowlesi growth inhibition assay for Plasmodium vivax Duffy-binding protein-based malaria vaccine development. Article Snippet: Briefly, cells were transfected following the manufacturer’s protocol using ExpiFectamineTM (Thermo Fisher Scientific), including the addition of enhancer 1 and enhancer 2 (Thermo Fisher Scientific) 18 h post-transfection. .. Supernatants were harvested seven days after transfection via centrifugation and mAbs were purified from culture supernatants using a 5 mL protein G column (Cytiva) in TrisBuffered Saline (TBS) on a Article Title: Isolation and molecular characterization of an FSK 2 -type dehydrin from Atriplex halimus. Article Snippet: Dehydrins form the group II LEA protein family and are known to play multiple roles in plant stress tolerance and enzyme protection.. They harbor a variable number of conserved lysine rich motifs (K-segments) and may also contain three additional conserved motifs (Y-, Fand S-segments).. In this work, we report the isolation and characterization of an FSK2-type dehydrin from the halophytic species Atriplex halimus, which we designate as AhDHN1. Article Title: Isolation and characterization of glycoprotein (CNP) isolated from Cocos nucifera L. nutshell and its immunomodulatory role on macrophage activation Article Snippet: The crude protein from Cocos nucifera L. nutshell was isolated and fractionized by GFC, yielding two protein peaks, CNP-F1 and CNP-F2 having a molecular weight of 43 kDa and 46 kDa, respectively as confirmed by MALDI-TOF.. MS/MS analysis followed by MASCOT search confirmed CNP-F1 was gibberellin-20-oxidase and CNP-F2 was serine/threonine kinase-11.. CNP-F1 (2.5 μg/mL) and CNP-F2 (0.5 μg/mL) upregulated the proinflammatory cytokines (IL-12, IFN-γ) release significantly (p < 0.01) with the prompt nitrite generation in murine peritoneal macrophages. Article Title: Molecular mechanism of CRISPR-SpyCas9 inhibition by AcrIIA26. Article Snippet: In the ongoing arms race between bacteria and phages, CRISPR-Cas systems and anti-CRISPR proteins (Acrs) have evolved to counteract one another.. Recently, AcrIIA26 from Streptococcus sp. was identified as a modulator of SpyCas9 activity, although its molecular mechanism remains unclear.. Here, we present the cryo-EM structure of the SpyCas9-sgRNA-AcrIIA26 complex, revealing a two-domain architecture. Lysis:Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania Article Snippet: Cells were harvested by centrifugation, and the pellet was resuspended in 10 mL lysis buffer containing 500 mM KCl, 20 mM Hepes, 5 mM imidazole, pH 7.5 and protease inhibitor cocktail without EDTA (Merck). .. After lysis by sonication (20 sec, 4 times), the soluble fraction was obtained by centrifugation (30,000 x g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. Article Snippet: purification Cas9 The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector, which contains two N- terminal and C- terminal His- tag (Gift from Fanny Boissier, INSERM U1212 CNRS 5320, University of Bordeaux), using the NcoI and EcoRI restriction sites. .. After lysis by sonication (20 sec, four times), the soluble fraction was obtained by centrifugation (30,000× g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His- Select Nickel Affinity Gel (Sigma) in a Sonication:Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania Article Snippet: Cells were harvested by centrifugation, and the pellet was resuspended in 10 mL lysis buffer containing 500 mM KCl, 20 mM Hepes, 5 mM imidazole, pH 7.5 and protease inhibitor cocktail without EDTA (Merck). .. After lysis by sonication (20 sec, 4 times), the soluble fraction was obtained by centrifugation (30,000 x g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. Article Snippet: purification Cas9 The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector, which contains two N- terminal and C- terminal His- tag (Gift from Fanny Boissier, INSERM U1212 CNRS 5320, University of Bordeaux), using the NcoI and EcoRI restriction sites. .. After lysis by sonication (20 sec, four times), the soluble fraction was obtained by centrifugation (30,000× g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His- Select Nickel Affinity Gel (Sigma) in a Centrifugation:Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania Article Snippet: Cells were harvested by centrifugation, and the pellet was resuspended in 10 mL lysis buffer containing 500 mM KCl, 20 mM Hepes, 5 mM imidazole, pH 7.5 and protease inhibitor cocktail without EDTA (Merck). .. After lysis by sonication (20 sec, 4 times), the soluble fraction was obtained by centrifugation (30,000 x g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. Article Snippet: purification Cas9 The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector, which contains two N- terminal and C- terminal His- tag (Gift from Fanny Boissier, INSERM U1212 CNRS 5320, University of Bordeaux), using the NcoI and EcoRI restriction sites. .. After lysis by sonication (20 sec, four times), the soluble fraction was obtained by centrifugation (30,000× g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His- Select Nickel Affinity Gel (Sigma) in a Article Title: Evaluation of the precision of the Plasmodium knowlesi growth inhibition assay for Plasmodium vivax Duffy-binding protein-based malaria vaccine development. Article Snippet: Briefly, cells were transfected following the manufacturer’s protocol using ExpiFectamineTM (Thermo Fisher Scientific), including the addition of enhancer 1 and enhancer 2 (Thermo Fisher Scientific) 18 h post-transfection. .. Supernatants were harvested seven days after transfection via centrifugation and mAbs were purified from culture supernatants using a 5 mL protein G column (Cytiva) in TrisBuffered Saline (TBS) on a Purification:Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania Article Snippet: Cells were harvested by centrifugation, and the pellet was resuspended in 10 mL lysis buffer containing 500 mM KCl, 20 mM Hepes, 5 mM imidazole, pH 7.5 and protease inhibitor cocktail without EDTA (Merck). .. After lysis by sonication (20 sec, 4 times), the soluble fraction was obtained by centrifugation (30,000 x g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. Article Snippet: purification Cas9 The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector, which contains two N- terminal and C- terminal His- tag (Gift from Fanny Boissier, INSERM U1212 CNRS 5320, University of Bordeaux), using the NcoI and EcoRI restriction sites. .. After lysis by sonication (20 sec, four times), the soluble fraction was obtained by centrifugation (30,000× g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His- Select Nickel Affinity Gel (Sigma) in a Article Title: One-Pot Three-Enzyme System for Production of a Novel Prebiotic Mannosyl-β-(1 → 4)-Fructose Using a d-Mannose Isomerase from Xanthomonas phaseoli . Article Snippet: The present supply of prebiotics is entirely inadequate to meet their demand.. To produce novel prebiotics, a Dmannose isomerase (XpMIaseA) from Xanthomonas phaseoli was first produced in Komagataella phaffii (Pichia pastoris).. XpMIaseA shared the highest amino acid sequence identity (58.0%) with the enzyme from Marinomonas mediterranea. Article Title: Evaluation of the precision of the Plasmodium knowlesi growth inhibition assay for Plasmodium vivax Duffy-binding protein-based malaria vaccine development. Article Snippet: Briefly, cells were transfected following the manufacturer’s protocol using ExpiFectamineTM (Thermo Fisher Scientific), including the addition of enhancer 1 and enhancer 2 (Thermo Fisher Scientific) 18 h post-transfection. .. Supernatants were harvested seven days after transfection via centrifugation and mAbs were purified from culture supernatants using a 5 mL protein G column (Cytiva) in TrisBuffered Saline (TBS) on a Article Title: Isolation and molecular characterization of an FSK 2 -type dehydrin from Atriplex halimus. Article Snippet: Dehydrins form the group II LEA protein family and are known to play multiple roles in plant stress tolerance and enzyme protection.. They harbor a variable number of conserved lysine rich motifs (K-segments) and may also contain three additional conserved motifs (Y-, Fand S-segments).. In this work, we report the isolation and characterization of an FSK2-type dehydrin from the halophytic species Atriplex halimus, which we designate as AhDHN1. Article Title: Molecular mechanism of CRISPR-SpyCas9 inhibition by AcrIIA26. Article Snippet: In the ongoing arms race between bacteria and phages, CRISPR-Cas systems and anti-CRISPR proteins (Acrs) have evolved to counteract one another.. Recently, AcrIIA26 from Streptococcus sp. was identified as a modulator of SpyCas9 activity, although its molecular mechanism remains unclear.. Here, we present the cryo-EM structure of the SpyCas9-sgRNA-AcrIIA26 complex, revealing a two-domain architecture. Affinity Chromatography:Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania Article Snippet: Cells were harvested by centrifugation, and the pellet was resuspended in 10 mL lysis buffer containing 500 mM KCl, 20 mM Hepes, 5 mM imidazole, pH 7.5 and protease inhibitor cocktail without EDTA (Merck). .. After lysis by sonication (20 sec, 4 times), the soluble fraction was obtained by centrifugation (30,000 x g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a Article Title: Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. Article Snippet: purification Cas9 The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector, which contains two N- terminal and C- terminal His- tag (Gift from Fanny Boissier, INSERM U1212 CNRS 5320, University of Bordeaux), using the NcoI and EcoRI restriction sites. .. After lysis by sonication (20 sec, four times), the soluble fraction was obtained by centrifugation (30,000× g, 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His- Select Nickel Affinity Gel (Sigma) in a Article Title: Isolation and molecular characterization of an FSK 2 -type dehydrin from Atriplex halimus. Article Snippet: Dehydrins form the group II LEA protein family and are known to play multiple roles in plant stress tolerance and enzyme protection.. They harbor a variable number of conserved lysine rich motifs (K-segments) and may also contain three additional conserved motifs (Y-, Fand S-segments).. In this work, we report the isolation and characterization of an FSK2-type dehydrin from the halophytic species Atriplex halimus, which we designate as AhDHN1. Transfection:Article Title: Evaluation of the precision of the Plasmodium knowlesi growth inhibition assay for Plasmodium vivax Duffy-binding protein-based malaria vaccine development. Article Snippet: Briefly, cells were transfected following the manufacturer’s protocol using ExpiFectamineTM (Thermo Fisher Scientific), including the addition of enhancer 1 and enhancer 2 (Thermo Fisher Scientific) 18 h post-transfection. .. Supernatants were harvested seven days after transfection via centrifugation and mAbs were purified from culture supernatants using a 5 mL protein G column (Cytiva) in TrisBuffered Saline (TBS) on a Saline:Article Title: Evaluation of the precision of the Plasmodium knowlesi growth inhibition assay for Plasmodium vivax Duffy-binding protein-based malaria vaccine development. Article Snippet: Briefly, cells were transfected following the manufacturer’s protocol using ExpiFectamineTM (Thermo Fisher Scientific), including the addition of enhancer 1 and enhancer 2 (Thermo Fisher Scientific) 18 h post-transfection. .. Supernatants were harvested seven days after transfection via centrifugation and mAbs were purified from culture supernatants using a 5 mL protein G column (Cytiva) in TrisBuffered Saline (TBS) on a SDS Page:Article Title: Isolation and molecular characterization of an FSK 2 -type dehydrin from Atriplex halimus. Article Snippet: Dehydrins form the group II LEA protein family and are known to play multiple roles in plant stress tolerance and enzyme protection.. They harbor a variable number of conserved lysine rich motifs (K-segments) and may also contain three additional conserved motifs (Y-, Fand S-segments).. In this work, we report the isolation and characterization of an FSK2-type dehydrin from the halophytic species Atriplex halimus, which we designate as AhDHN1. Filtration:Article Title: Isolation and characterization of glycoprotein (CNP) isolated from Cocos nucifera L. nutshell and its immunomodulatory role on macrophage activation Article Snippet: The crude protein from Cocos nucifera L. nutshell was isolated and fractionized by GFC, yielding two protein peaks, CNP-F1 and CNP-F2 having a molecular weight of 43 kDa and 46 kDa, respectively as confirmed by MALDI-TOF.. MS/MS analysis followed by MASCOT search confirmed CNP-F1 was gibberellin-20-oxidase and CNP-F2 was serine/threonine kinase-11.. CNP-F1 (2.5 μg/mL) and CNP-F2 (0.5 μg/mL) upregulated the proinflammatory cytokines (IL-12, IFN-γ) release significantly (p < 0.01) with the prompt nitrite generation in murine peritoneal macrophages. Chromatography:Article Title: Molecular mechanism of CRISPR-SpyCas9 inhibition by AcrIIA26. Article Snippet: In the ongoing arms race between bacteria and phages, CRISPR-Cas systems and anti-CRISPR proteins (Acrs) have evolved to counteract one another.. Recently, AcrIIA26 from Streptococcus sp. was identified as a modulator of SpyCas9 activity, although its molecular mechanism remains unclear.. Here, we present the cryo-EM structure of the SpyCas9-sgRNA-AcrIIA26 complex, revealing a two-domain architecture. Ion Exchange Chromatography:Article Title: Molecular mechanism of CRISPR-SpyCas9 inhibition by AcrIIA26. Article Snippet: In the ongoing arms race between bacteria and phages, CRISPR-Cas systems and anti-CRISPR proteins (Acrs) have evolved to counteract one another.. Recently, AcrIIA26 from Streptococcus sp. was identified as a modulator of SpyCas9 activity, although its molecular mechanism remains unclear.. Here, we present the cryo-EM structure of the SpyCas9-sgRNA-AcrIIA26 complex, revealing a two-domain architecture. |
